Composite

Part:BBa_K1976025:Design

Designed by: Patrick Kunzmann   Group: iGEM16_TU_Darmstadt   (2016-10-13)


Orthogonal pair for amber supression with OMT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1153
    Illegal BamHI site found at 1159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1185
    Illegal NgoMIV site found at 1645
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Synthesis feasible by IDT, no amber stop codon for termination


Source

Methanocaldococcus jannaschii (optimized via directed evolution)

References